Author's response to reviews Title: Evaluation of bleach-sedimentation for sterilizing and concentrating M. tuberculosis in sputum samples Authors:
نویسندگان
چکیده
R2: “Tuberculosis diagnosis by sputum smear microscopy is insensitive.” Insert ‘relatively’ before ‘insensitive’ R3: In Abstract, background section, on the first line, authors could replace the term "insensitive" by "low sensitive" (sic). The word ‘relatively’ has been added before ‘insensitive’ as recommended by R1. R2: The remaining 25 sputum samples were used to evaluate this technique quantitatively by making triplicate conventional direct-smears, then subjecting the remaining sputum to this bleach-sedimentation protocol, after which additional triplicate slides were made Why triplicate? Why not single? Need explanation. This has been clarified with the addition of the sentence: “Slides from each sample both before and after bleach-sedimentation were prepared in triplicate to increase the precision with which the concentrations of stainable acid-fast bacilli were quantified.”
منابع مشابه
Evaluation of bleach-sedimentation for sterilising and concentrating Mycobacterium tuberculosis in sputum specimens
BACKGROUND Bleach-sedimentation may improve microscopy for diagnosing tuberculosis by sterilising sputum and concentrating Mycobacterium tuberculosis. We studied gravity bleach-sedimentation effects on safety, sensitivity, speed and reliability of smear-microscopy. METHODS This blinded, controlled study used sputum specimens (n = 72) from tuberculosis patients. Bleach concentrations and expos...
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